This second edition has been extended to include new information and developments in the preservation of fungi but maintains the coverage of methodology as presented in the first edition published in 1983. The book enables readers to select suitable preservation procedures for their purposes, gives information on the growth and maintenance of fungi and provides the background to manage a collection of microorganisms both safely and effectively. The methods described are those in operation or experimented upon at IMI, and cover the simple to the more complex techniques. The methods listed are of use in all situations where living cultures of fungi are required, from teaching and research to industry and technology.