This publication is part of the lAEA's continuing efforts to transfer technology and to contribute to capacity building by providing information on the theoretical background and practical application of state of the art methodologies for assessing human zinc metabolism to better understand absorption, dietary bioavailability and nutritional requirements. It reviews the role of zinc in human nutrition and the application of stable isotope techniques to evaluate nutritional interventions. Advice is given on planning a study, administering isotopes, preparing and analysing samples, and calculating physiological end points. The publication was developed with input from international experts and is intended for nutritionists, analytical chemists and other professionals interested in the application of stable isotope techniques to evaluate human zinc nutrition and metabolism.